Germany will be without striker Cacau when they face England in the second round of the World Cup in Bloemfontein on Sunday.
Cacau, 29, played a part in all three of Germany's group games but suffered an abdominal muscle strain during training on Friday, ruling him out of the clash against England.
"This injury means that he cannot play against England,'' manager Oliver Bierhoff told The German FA's official wesbite. "We are working to ensure he will be fit for the next game, we are confident he will be.''
Injury doubts Bastian Schweinsteiger and Jerome Boateng are set to undergo fitness tests on Saturday and Bierhoff is hopeful they will be fit to face Fabio Capello's side.
"We will see if their injuries have recovered enough for them to take a full part in training with the rest of the team,'' he said.
Meanwhile, FIFA secretary general Jerome Valcke has confirmed that there will be "additional security measures'' in place for the second round match.
"We have agreed with the South African Police Service that there will be a number of additional security measures, a few which you will see and a few that you will not. Kicking off at 4pm (local time) makes things easier than a night game as well.''
LOC chief executive Danny Jordaan added: "Not just Bloemfontein but the whole country is waiting for this match. We have had a meeting with the police to talk about security and I'm sure fans will go there and have a wonderful time.
"A huge contest is expected and we look forward to this event. It came earlier on than expected but this is World Cup.''