Cheltenham's fringe players will have the chance to impress against Plymouth in the Johnstone's Paint Trophy after a number of injuries to Mark Yates' squad.
Steve Elliott and Craig Braham-Barrett will not be available, Matt Richards has a hamstring problem and remains a doubt while Troy Brown is still out.
One player who may return to the bench is Jamie Cureton. The experienced forward has been sidelined with a shoulder problem he sustained when opening his account for the club on the opening day.
Yates has called for his players to stand up and be counted after their 4-1 defeat to Bury on Saturday.
Plymouth could hand debuts to two new signings for the visit to Whaddon Road.
Left-back Jamie Reckord, who arrived on loan from Wolves, and Algerian midfielder Hamza Bencherif, who was a free agent, have both been signed in time to feature.
Striker Matt Lecointe underwent a cruciate knee ligament operation last week and faces a lengthy recovery period while this match comes too soon for Paul Wotton as he recovers from his broken ribs.
Argyle make the trip looking to bounce back from Saturday's 2-1 defeat at Morecambe.