Defender Stephen Black is nursing a knee problem as Annan prepare to play host to Highland League Formartine looking to progress to the semi-finals of the Ramsdens Cup for the third time in five years.
The Galabank side also have doubts over goalkeeper Alex Mitchell as well as Steven Swinglehurst and Chris Jardine, with a loan move for Rangers' Andrew Mitchell put on hold after the defender was called into Northern Ireland's Under-21 squad.
Black and Gold manager Jim Chapman said: "Hopefully we can get as strong as side as possible onto the park. It is fantastic opportunity for both sides to progress and we have to be at our best to get through."
History-makers Formartine head south without defender Mark Smith as they look to continue the finest run a Highland League side has ever made in the competition.
Smith is carrying a groin problem and, after Stuart Soane joined Forres Mechanics on loan, it leaves manager Steve Paterson with a squad of just 16 to choose from.
Paterson said: "We are the underdogs as Annan are a good side, as they showed when they knocked Morton out in the first round. We have done our research but I have told my players that what they do in the game will determine the outcome.
"It would be great to keep the Highland League flag flying."