Annan include summer signings Scott Davidson and Kenny McKay against visitors Morton.
But fellow new boy Stephen Black will miss out after picking up an ankle injury.
Galabank boss Jim Chapman said: ``Morton are a good side and this will be a difficult tie to win but our young players will pick up a lot from playing against them.
``However I don't want them standing back and learning, I want us to be working as hard as we can to pull off a terrific result.''
Morton captain Mark McLaughlin is the only doubt for the Cappielow side after he suffered a jaw injury in last weekend's Renfrewshire Cup win over St Mirren.
Everyone else in manager Allan Moore's new-look squad is available for selection.
Moore said: ``We have looked good in pre-season and have the potential to be an exciting side, however having the right attitude on the pitch will be needed to see off Annan.
``They will be out to create a shock and we have to ensure that does not happen.''