Gillingham boss Martin Allen could hand debuts to four new players when they host Colchester.
Striker Cody McDonald is likely to start in attack following his summer switch from Coventry.
Fellow forward Adebayo Akinfenwa is also in contention after re-signing for the club having been released by Northampton.
Striker Antonio German and midfielder Amine Linganzi are in contention as the Gills prepare for life in League One after a five-year absence.
Colchester boss Joe Dunne has four new players at his disposal.
Chelsea loanee Daniel Pappoe is expected to line up in defence while Ryan Dickson should also feature at the back along with former Arsenal trainee Craig Eastmond.
Teenage full-back Mason Spence may also be involved while Sanchez Watt and Sam Walker returned to the U's over the summer.
Winger Watt had a loan spell cut short by injury last season but has now agreed a permanent switch from Arsenal, while Chelsea goalkeeper Walker is expected to start in goal after agreeing a new loan deal.