Josh Falkingham should return to Dunfermline's line-up against Aberdeen at East End Park after the midfielder missed Saturday's 4-0 win over Livingston with a virus.
Chris Kane's ankle injury continues to keep him sidelined.
Assistant manager Gerry McCabe believes his side can see off their SPL opponents, saying: ``We defeated Aberdeen 3-0 at East End Park in our best home performance of last season, so we can go into the game with nothing to fear.
``They will be tough opponents but being at home is always a good draw to get. They had been struggling to score goals but got three at the weekend, but they also conceded three and that will be a worry for them.''
Meanwhile, Aberdeen manager Craig Brown has urged his players to seize a ``great opportunity'' for trophy success.
Four of the seven Clydesdale Bank Premier League teams involved in the second round lost to lower-league opposition, and Brown wants his team to capitalise.
Brown, who has led his team in three Hampden semi-finals since arriving from Motherwell in December 2010, said: ``We've got to approach every game with the determination to be successful because we want to be successful on all fronts.
``The SPL is obviously the bread and butter, the priority, but there's a great opportunity for all clubs and, particularly I would say ours at the moment, in this competition, because some of the SPL, I think four are out already, and other teams who could cause a problem, Motherwell and Rangers, we're going to lose one of them.
``So there's a great opportunity for us all, for those left, and we want to seize that opportunity so we'll do our utmost at Dunfermline to progress to the quarter-final.''
The Dons face one of the toughest tests of the SPL teams left though, with the clash seeing them go up against a Dunfermline side led by Jim Jefferies who are unbeaten in the Irn-Bru First Division following their relegation last season.
Brown said: ``Jim's a wonderful manager and you can be sure he's saying to his players that this is an opportunity to prove themselves.
``This is a great opportunity for them to prove that they were deserving of being in the SPL.
``Jim's made a couple of good signings and strengthened what was a Dunfermline team that gave us problems last year.
``So I think it's a stronger-looking Dunfermline team right now than the one that was relegated.''
Brown could make changes following Sunday's 3-3 draw with SPL leaders Motherwell.
``We'll probably freshen the team but I wouldn't say we'll make massive changes,'' he said.
``We haven't yet decided on the team because one or two have bumps and bruises.
``Wee Ryan Fraser, for example, didn't train today (Tuesday). It's not surprising that he's got a few bruises after every game we find, but he's such a brave guy, he never lies down, he gets up and gets on with it, but he's requiring treatment at the moment.''