Nathan Byrne will be suspended for Swindon's Johnstone's Paint Trophy Southern Area semi-final at home to Stevenage.
The full-back saw red in Town's goalless draw at Crawley and, having missed the 3-1 victory over Carlisle, completes a two-match ban against Boro.
Manager Mark Cooper is not expected to boast any other injury or suspension concerns as Town return to action for the first time since their win over the Cumbrians at the end of November.
Swindon will be looking for a repeat of their narrow 1-0 League One success over Stevenage when the two sides met at the County Ground in August.
Stevenage midfielder Michael Doughty will be hoping for more game time during the tie.
The on-loan QPR midfielder was substituted just 12 minutes into Saturday's 4-0 FA Cup win over non-league Stourbridge having already been booked, with manager Graham Westley fearing he may see red.
Defender Ben Chorley was on the bench for the first time this season after finally recovering from groin and thigh problems so he could be involved again.
Striker Darius Charles is therefore the only injury absentee with ankle ligament damage.