Swindon manager Mark Cooper could shuffle his pack ahead of the Johnstone's Paint Trophy tie against League Two Plymouth.
Cooper admitted to rushing players back from injury after Saturday's 1-0 victory over Tranmere. Nathan Byrne, Nathan Thompson and Nile Ranger all started the match despite carrying knocks. They could be rested for the clash.
Ryan Harley and Ryan Mason are expected to miss out once again with knee and thigh problems respectively.
Swindon, who reached the second round courtesy of a bye, have already gone one better than last season, when they fell at the first hurdle after defeat by Oxford.
Plymouth boss John Sheridan could choose to stick with the bulk of the side that took the field for Saturday's Devon derby defeat to Exeter.
Sheridan was pleased with Argyle's performance and felt they deserved better, although he did concede they "were all over the place" in the dying stages of the 3-1 defeat.
Durrell Berry was available for the game after recovering from a virus, but Sheridan kept faith with Curtis Nelson, while teenager Tyler Harvey is also in contention for a rare start.
Jake Cole started in goal in the first-round victory over Cheltenham and could come in for number one Luke McCormick.