Queen of the South are sweating on the fitness of defender Chris Higgins and striker Michael Paton for the visit of Scottish League Cup holders St Mirren to Palmerston Park.
The Doonhamers lost for the first time this season when Hamilton beat them 2-0 on Saturday and manager Jim McIntyre has welcomed a quick return to action.
McIntyre said: "Facing the holders just a few days after losing is the perfect tonic as everyone is desperate to get going again.
"I may need to freshen the side up after a demanding run of games and I will wait to see what options I have before deciding the team."
Meanwhile, St Mirren manager Danny Lennon has warned his players their status as holders will make them even more of a target for the home side.
The third-tier champions and Ramsdens Cup holders sprang several surprise results last term and Lennon knows St Mirren will have to be at their best to avert a second-round shock at Palmerston.
Lennon said: "Winning the cup last season was such a highlight for everyone associated with this club. It was only the fourth time in 134 years that this club has won a national trophy.
"The celebrations were great but nobody hands out trophies for free. We know how hard we worked last year to lift that trophy and we need to work hard to have a chance of doing it again.
"It's a massive one for us. Everyone wants to knock out the holders of a trophy. Look at last season when the holders, Kilmarnock, were put out by Stenhousemuir. We certainly don't want to suffer a similar fate.
"We will not be able to drop our standards for even a second against Queen of the South. We need to put in a top performance."
Saints have shipped three goals in each of their two Scottish Premiership away games this season - against Ross County and Inverness - and even more when they played on the new artificial surface at Palmerston before the campaign started.
"We played them in pre-season and got beat 4-2," he said. "The positive from that is that we have already got a feeling for their pitch and the surroundings.
"We know they have got great quality and they play well as a team. They certainly like cup competitions themselves having beaten Hibs in this competition and won a cup, beating teams like Rangers and Partick Thistle. They will be hungry."
Lennon has no new fitness concerns for the clash.