Ayr striker Michael Moffat is due to start his six-game ban imposed for breaching football betting rules against Brechin, although the Somerset Park club are considering an appeal.
If they decide to contest the ban he will be free to face the Glebe Park side, with the Honest Men hoping Brian Gilmour will also be available despite suffering from a toe injury.
Player-manager Mark Roberts said: "It was a devastating result for us and for Michael, however I will not make any more comment.
"Brechin are a top side who will be disappointed at where they sit in the table. That makes them dangerous opponents."
Brechin welcome back Jonny Brown from a ban but Greg Cameron will not play due to a hamstring injury.
Manager Ray McKinnon is hoping to add at least one new face ahead of kick-off as he looks for his men to build on last Saturday's 1-0 success over Arbroath that lifted them up to seventh in the table.
McKinnon said: "It was a very solid performance at Arbroath where we got in front and then were resilient.
"We will need the same approach at Somerset Park as Ayr won handsomely last weekend and will be looking to put a lot of space between them and us in the play-off race."