Raith will list an unchanged squad from the one that defeated Queen's Park 6-0 last weekend when they welcome Hamilton.
Player/manager Grant Murray has no injury or suspension worries to contend with, but is anticipating a much closer scoreline in this game.
"Our boys were pretty ruthless and when it was 3-0 and Queen's Park had a man dismissed their heads went down a bit," he said.
"It was good to see our strikers scoring as that is always good for confidence.
"Hamilton will be a different proposition as they won well last weekend and their manager Alex Neil will have them fired up to start their league campaign with a bang."
Hamilton are without three as they head to Stark's Park with Martin Canning banned, Louis Longridge out with a foot injury and Jonny Routledge missing after picking up a knee problem.
The New Douglas Park side started last season with a defeat at Raith and Neil is looking for better this time out.
"It would be terrific to pick up the three points this time and give ourselves a terrific lift going into the league season," he said.
"It is always a hard game there but going into it without three key players makes it all the more difficult."