Raith boss Grant Murray has a fully-fit squad ahead of their meeting with Queen's Park.
Rovers will be looking to knock a League Two side out of a cup competition for a second successive week following their recent 2-1 win over Stirling.
Murray said: "Having a full squad at this time of year is a real luxury so we will be on the same lines as last week.
"We played well to defeat Stirling Albion last week and are now looking to do the sameagainst Queen's Park. They will want to play the good football that they are well known for but if we go about our business in the correct manner again we will get through."
Queen's Park have defensive concerns ahead of the trip with Paul Gallacher sidelined with a knee injury.
Fellow defender Scott Gibson is also missing with a groin problem while Bernard Coll is also unavailable.
Head coach Gardner Speirs is hoping Jamie Brough can overcome the virus that saw him miss training on Thursday in time to deputise at the back.
Speirs said: "I may be able to bring in a new player before kick-off but our defensive options are greatly reduced. However, we will get on with things and look to take the pressure off our defence by being positive."