Livingston skipper Liam Fox has returned to full training after six months out but will not be ready to face Cowdenbeath.
The Lions will also be without long-term injury victims Jason Talbot and Kyle Jacobs.
But the rest of the squad are fit after last weekend's first home win of the season, 5-0 against Dumbarton.
First-team coach Gareth Evans said: ``We go into this game with increased confidence but we know that we will need to work hard to win.
Cowdenbeath are a well-organised side who have adapted to the First Division well.
``They have 10 points already but we are targeting a win and a move above them in the table.''
Cowdenbeath striker Zeph Thomas is away on Caribbean Cup duty with St Kitts & Nevis.
Long-term absentee Liam Cusack and Spanish signing Pablo Navas, who has a hip problem, are also ruled out.
Scott Linton and Marc McKenzie have missed training through illness and manager Colin Cameron is giving them until Saturday lunchtime to prove their fitness.
Cameron said: ``Livingston will be flying after a great win and I want to be facing them with as strong a side as possible so I will wait on Scott and Marc.
``They are great at passing the ball about but we are not going there to get picked apart. We will look to pass the ball about ourselves.''