All kick-off times in GMT.
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- Wed, Jun 19, 2013
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| FT | Montreal Impact | 2 - 0 | Houston |
| FT | Chicago | 2 - 1 | Colorado |
| FT | Vancouver Whitecaps | 3 - 1 | Chivas USA |
| FT | Los Angeles | 0 - 0 | Portland Timbers |
| FT | Talleres de Córdoba | 1 - 0 | Newell's Old Boys |
| FT | Atletico Rafaela | 0 - 3 | San Lorenzo |
| FT | All Boys | 3 - 1 | Boca Juniors |
| FT | Sport Huancayo | 4 - 1 | José Gálvez |
| FT | León de Huánuco | 1 - 0 | Real Garcilaso |
| FT | Juan Aurich | 1 - 2 | Universitario de Deportes |
| FT | San Martin | 0 - 0 | César Vallejo |
| FT | Alianza Lima | 1 - 0 | Sporting Cristal |
| FT | General Díaz | 1 - 0 | Sp. Carapeguá |
| FT | Deportivo Capiatá | 1 - 1 | Sol de América |
| FT | Rubio Ñú | 1 - 0 | Nacional |
| FT | Guaraní | 2 - 0 | Cerro Porteño |
| FT | Sportivo Luqueño | 0 - 0 | Cerro Porteño de Franco |