All kick-off times in GMT.
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- Sun, Mar 10, 2013
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| FT | Athletic Bilbao | 1 - 0 | Valencia |
| FT | Levante | 0 - 0 | Getafe |
| FT | Celta Vigo | 1 - 2 | Real Madrid |
| FT | Atletico Madrid | 0 - 1 | Real Sociedad |
| FT | Villarreal | 2 - 1 | Sporting Gijon |
| FT | Girona | 2 - 1 | Huesca |
| FT | Racing Santander | 2 - 1 | Murcia |
| FT | AD Alcorcon | 2 - 1 | Recreativo Huelva |
| FT | Atalanta | 2 - 1 | US Pescara |
| FT | Cagliari | 3 - 1 | Sampdoria |
| FT | Chievo Verona | 2 - 0 | Napoli |
| FT | Juventus | 1 - 0 | Catania |
| FT | Palermo | 1 - 2 | Siena |
| FT | Parma | 4 - 1 | Torino |
| FT | Internazionale | 0 - 1 | Bologna |
| FT | Lazio | 0 - 2 | Fiorentina |
| FT | FC Erzgebirge Aue | 1 - 0 | Dynamo Dresden |
| FT | FC Ingolstadt 04 | 2 - 1 | VfL Bochum |
| FT | Hertha Berlin | 4 - 2 | MSV Duisburg |
| FT | Roda JC Kerkrade | 0 - 1 | Feyenoord Rotterdam |
| FT | Ajax Amsterdam | 3 - 0 | PEC Zwolle |
| FT | FC Utrecht | 1 - 0 | RKC Waalwijk |
| FT | Twente Enschede | 0 - 1 | Vitesse Arnhem |
| FT | CD Nacional de Madeira | 1 - 1 | Rio Ave |
| FT | Moreirense | 1 - 1 | Olhanense |
| FT | Paços de Ferreira | 1 - 1 | Beira Mar |
| FT | Benfica | 5 - 0 | Gil Vicente |
| FT | Spartak Moscow | 3 - 1 | Terek Grozny |
| FT | FK Rubin Kazan | 1 - 0 | Zenit St Petersburg |
| FT | Mordovia Saransk | 2 - 0 | Anzhi Makhachkala |
| FT | Karabukspor | 0 - 0 | Eskisehirspor K |
| FT | Sivasspor | 2 - 1 | Antalyaspor |
| FT | Elazigspor | 1 - 0 | Orduspor |
| FT | Fenerbahce | 4 - 1 | Bursaspor |
| FT | FC Xanthi | 0 - 1 | Giannina |
| FT | Aris Salonika | 4 - 0 | Levadiakos |
| FT | Veria | 0 - 0 | OFI Crete |
| FT | Olympiakos | 3 - 0 | AEK Athens |
| FT | Antofagasta | 1 - 3 | O'Higgins |
| FT | Rangers de Talca | 1 - 0 | Colo Colo |
| FT | Universidad Católica | 2 - 3 | Santiago Wanderers |
| FT | El Tanque Sisley | 2 - 0 | Fénix |
| FT | Juventud | 4 - 1 | Cerro |
| FT | Racing (U) | 1 - 1 | River Plate |
| FT | Wanderers | 1 - 2 | Danubio F.C. |
| FT | Nacional | 2 - 1 | Central Español |
| FT | Alianza Petrolera | 1 - 2 | Envigado |
| FT | Atlético Huila | 1 - 1 | Atlético Junior |
| FT | Atlético Nacional | 3 - 3 | Patriotas F.C. |
| FT | Independiente Santa Fe | 3 - 1 | Deportivo Cali |
| FT | Once Caldas | 2 - 1 | Independiente Medellín |
| FT | Melgar FBC | 1 - 2 | Inti Gas Deportes |
| FT | Unión Comercio | 1 - 1 | Club Sportivo Cienciano |
| FT | Real Garcilaso | 0 - 0 | León de Huánuco |
| FT | Universitario de Deportes | 1 - 0 | Juan Aurich |
| FT | Rubio Ñú | 0 - 1 | Sportivo Luqueño |
| FT | Sol de América | 1 - 1 | Cerro Porteño de Franco |
| FT | Cerro Porteño | 0 - 1 | Deportivo Capiatá |
| FT | Petrolero | 1 - 1 | Universitario de Sucre |
| FT | Real Potosí | 3 - 1 | La Paz |
| FT | Bolívar | 5 - 3 | Wilstermann |
| FT | Oriente Petrolero | 3 - 2 | Blooming |
| FT | Perez Zeledon | 1 - 1 | Club Sport Uruguay |
| FT | Limon | 1 - 1 | Puntarenas |
| FT | San Carlos | 1 - 1 | Belen |