Socceroos duo Tommy Oar and Mile Jedinak have been omitted from the squad set to face Romania in an international friendly.
Both players were expected to gather game time as Australia prepares for its next phase of World Cup qualification but injury has forced both to pull out of the match.
Crystal Palace's Jedinak is nursing a fractured cheekbone and broken nose, while Oar is still recovering from a hamstring injury.
Melbourne Victory's Mark Milligan has been called up as a replacement.
The Socceroos head into the match looking to fine-tune their plans for the upcoming World Cup qualifying match against Oman next month.
And with competition across the park beginning to heat up, Wednesday's game is shaping up as another opportunity for players to force their way into the reckoning of head coach Holger Osieck.
One of those battles will be in the left-back position where youngsters Jason Davison and Michael Zullo are hoping to cement their name in Osieck's starting XI.
"Right now Michael and I are here in camp and I think we're the two front runners for the role," Davison told theworldgame.com.au. "Maybe in the next few months other players will also emerge but for now us two have to battle it out and we'll see who takes the position.
"The boss (Osieck) has shown that when I am playing he is willing to give me a chance.
"I'll give it all at training and just see if that's good enough to get me in against Romania." Davison endured a horror debut to his Socceroos career, scoring an own goal in the 3-1 loss to Scotland.
But the match against Romania offers the 21-year-old a chance for redemption and the opportunity to stake a claim for Brazil 2014.
Revised Socceroos squad: Mark Bresciano, Alex Brosque, Robert Cornthwaite, Jason Davidson, Adam Federici (GK), James Holland, Brett Holman, Robbie Kruse, Mathew Leckie, Ryan McGowan, Matt McKay, Mark Milligan, Lucas Neill, Nikita Rukavytsya, Mark Schwarzer, Luke Wilshire, Rhys Williams, Michael Zullo.