Lee Cattermole is likely to miss Sunderland's trip to Wigan on Saturday after suffering an injury setback in training.
Cattermole, 24, returned from a knee problem in Tuesday's FA Cup third round replay defeat at home to Bolton, playing the final 15 minutes.
However, manager Martin O'Neill said the former Latics midfielder had suffered a setback and was a doubt to face his old club.
"We have a number of injury problems ahead of the Wigan game," O'Neill told the Sunderland Echo. "Lee Cattermole has had a bit of a set-back.
"He trained for the game yesterday - we were trying to get a bit more fitness into him and the new lads who have arrived - and he had a bit of a setback.
"We are hoping it's not as bad as before, but I would say he would be doubtful for tomorrow."
The Sunderland captain signed a new contract in October, but his season has been blighted by injury and he has made only nine Premier League appearances this season.
O'Neill said he remained frustrated by the 2-0 defeat to Championship Bolton, adding: "Sometimes I think that moving on from a game quickly, just like that, has never been something in my make-up.
"Even as a player, I would be thinking about games some days later. It was a chance for us to get through in midweek, a chance for us to meet up with Everton in the next round.
"But maybe sometimes I'm too hard because the fact is that in the league we've done exceptionally well of late. Tomorrow is a chance to continue that good form and put the midweek disappointment behind us."