United and Barca top groups
Pablo Hernandez fired Valencia ahead at Old Trafford after rounding off a swift Los Che counter-attack.
But the Manchester United fans were in for a treat as they witnessed the rare sight of Anderson scoring; his equaliser just reward for a second half of dominance for United.
Anderson's goal - only his second for United and first at Old Trafford - ensured Sir Alex Ferguson's men finished ahead of Valencia in Group C
Forty-year-old Twente goalkeeper Sander Boschker gifted Tottenham the lead in the Netherlands after letting the ball slip under his foot.
Spurs took the lead on three occasions in the game, Jermain Defoe netting two goals, as Twente battled back to a 3-3 draw. But it was enough for the English side to top Group A.
Barcelona's towering young centre-back Andreu Fontas scored to help the Catalans to a 2-0 victory over Rubin Kazan.
Sebastian Prodl opened the floodgates for Werder Bremen, scoring the first of three goals as they beat an under-strength Inter Milan side 3-0 in Germany.
Inter striker Samuel Eto'o cut a forlorn figure during his side's embarrassing defeat.
The Champions League has continued to provide a welcome respite in what has been a tough season for Schalke.
Schalke's result made the result in Lyon's clash with Hapoel Tel-Aviv futile. Lisandro Lopez scored for the French side as they played out a 2-2 draw.
FC Copenhagen made history by becoming the first Danish side to reach the Champions League knockout stage, beating Panathinaikos to finish second.
Kenny Miller netted his 19th goal of the season to put Rangers ahead but Bursaspor fought back to earn a 1-1 draw.