Emile Sinclair scored twice as League Two minnows Macclesfield topped the bill on a night of Carling Cup upsets with a 2-0 win over Championship Hull at the KC Stadium.
Sinclair, a 23-year-old former Nottingham Forest trainee, opened the scoring in the 17th minute and completed a famous victory for the visitors in the 59th minute when he netted his second.
Fellow League Two sides Morecambe, Shrewsbury, Northampton and Barnet joined Macclesfield in overcoming Championship opposition away from home in normal time.
Mark Hughes scored the only goal of the game on the half-hour as Barnet stunned Portsmouth at Fratton Park while Ben Tozer and Paul Turnbull cancelled out Jay Emmanuel-Thomas' excellent early strike to give Northampton a 2-1 win at Ipswich.
Shrewsbury stormed into a 3-0 lead over Derby at Pride Park through James Collins and a Marvin Morgan double, then withstood a second-half fightback to win 3-2, while Danny Carlton and Kevin Ellison gave Morecambe a 2-0 win at Barnsley.
Newly-promoted League One side Bury joined in the upsets as they coasted to a 3-1 win over Coventry at Gigg Lane. Andy Bishop cancelled out Roy O'Donovan's early strike before Ryan Lowe hit a match-winning double late on.
Simon Grayson's Leeds narrowly avoided embarrassment in their West Yorkshire derby with Bradford at Elland Road, twice falling behind before Ramon Nunez's second goal of the night in the 75th minute edged them a 3-2 win.
Jeffery Schlupp scored a hat-trick as Leicester eased to a 4-1 win at Rotherham while Southampton beat Torquay by the same score. An Ashley Barnes penalty saw Brighton home against Gillingham.
Other Championship winners in normal time were Millwall, who won 1-0 at Plymouth, Doncaster, who beat Tranmere 3-0, and Middlesbrough, who coasted to a 3-0 win at Walsall with a hat-trick from Marvin Emnes.
League Two Hereford caused a minor upset as Yoann Arquin scored in injury time to overcome League One Brentford at Edgar Street.
Bournemouth were the biggest winners of the night as they thrashed Dagenham and Redbridge 5-0 while MK Dons and Huddersfield each scored four away from home to beat Cheltenham and Port Vale respectively.
Scunthorpe eased to a 2-0 win at Accrington Stanley, Exeter beat Yeovil by the same scoreline and late goals from Neil Mellor and Iain Hume helped Preston scrape a 3-2 home win over Crewe.
Nottingham Forest narrowly avoided becoming another Championship casualty after a thrilling city derby with Notts County.
Lee Hughes put County, who had led through Mike Edwards' 16th-minute strike only to be pegged back to 2-2 after 90 minutes, back in front in extra-time but Wes Morgan equalised in the 120th minute and Forest held their nerve to win 4-3 on penalties.
Burnley also avoided embarrassment as they pulled away from League Two Burton in the extra half-hour. Taken to 3-3 after 90 minutes, the Clarets eventually won 6-3 with Jay Rodriguez scoring four.
Stevenage put up a brave fight at home to Peterborough but went down 4-3 after extra-time with Lee Tomlin's 120th-minute penalty enough to win it for the Championship club.
Sheffield United, Carlisle, Leyton Orient and Wycombe all held their nerve to advance on penalties while a late Dwayne Mattis own goal saw Rochdale through at the expense of Chesterfield.