Manchester United manager Sir Alex Ferguson has expressed his disappointment at Owen Hargreaves again breaking down with a knee injury.
The England midfield suffered the setback before the 2-1 defeat by Zenit St Petersburg in the UEFA Super Cup.
Hargreaves was hoping to get some minutes on the clock but pulled up in training last night.
Ferguson said: 'He said he felt his knee and that he was not fit enough to play.
'There is nothing you can to do about it. If a player declares himself unfit you have to accept that.
'We'd have liked to play him and get him back into the run of things and playing football again.
'To be honest, he has not played enough football in the last year. He'd like to get back and it is disappointing.'
Hargreaves appeared to be in line for an England call for the forthcoming World Cup qualifiers against Andorra and Croatia - even though Ferguson voiced his disapproval at the prospect earlier this week.
Instead he is now certain to sit out the back-to-back matches for Fabio Capello's side.
Goals from Pavel Pogrebnyak and Danny gave the UEFA Cup holders a memorable victory.
Pogrebnyak scored a powerful header at the back post to leave United rattled a minute before half-time.
Then Danny, a shrewd investment at 30 million euros (£24million), provided a touch of class in the 59th minute after shrugging off Rio Ferdinand.
Nemanja Vidic pulled a goal back but United never really got going and Paul Scholes was sent off in the final minute for a deliberate handball which led to his second booking.
The midfielder will now miss United's Champions League game against Villarreal on September 17.
'The sending off was unfortunate, it was an instinctive thing,' said Ferguson.
'The fact he had already been booked meant he had to go, unfortunately.
'I cannot deny that Zenit deserved to win the game. I think that they probably just shaded it a little from us.'
Ferguson was pleased to see Ji-sung Park return to action after a knee injury.
'It was first game for three or four months and his first step forwards after his knee operation,' he said.
'I think with Ji-sung Park you get a good player with a good understanding of the game, good movement and he did his job okay.
'We played better when we were 2-0 down and made some good chances and when we scored with something like 17 minutes to go, we still found time to recover and get back into the game.
'But the game was running away from us a little bit.'