UEFA on Thursday fined Lazio £32,500 ($52,000) for the improper conduct of fans during last month's Europa League tie against Tottenham at White Hart Lane.
Loud monkey chanting was heard to come from the away fans and was directed at Jermain Defoe, Aaron Lennon and Andros Townsend during the Group J clash, which ended 0-0 after three Spurs goals were ruled out.
Lazio has up to three days to appeal against the fine if they feel the findings of the panel are unfounded.
The sanctions, coupled with several other ongoing racial abuse cases, have come at a bad time for UEFA as Champions League and Europa League matches next week will be used to showcase activities organized by UEFA and the equality group Football Against Racism in Europe.
Disciplinary action has also been brought against Serbia following what appeared to be racist chanting from their fans during the Euro 2013 Under-21 Championship play-off against England on Tuesday night.
Spurs winger Danny Rose, who is currently on loan at Sunderland, was sent off after the final whistle for his reaction to the abuse while Townsend was an unused substitute.
Rose was offered support by Spurs boss Andre Villas-Boas Thursday.
"It's extremely difficult for Danny," he said. "He was disappointed by that happening, by the situation which he had to live with, the atmosphere he was confronted with.
"I told Daniel I support him completely in this situation. He felt abused and this is obviously a serious matter which at the moment is in the hands of both FAs."
Lazio currently top Group J after picking up four points from their first two games and host Spurs at the Stadio Olimpico on Nov. 20.