Former Lyon midfielder Juninho Pernambucano is to face the Brazilian Sporting Justice Tribunal (STJD), along with two other players, for failing to comply with drug-testing regulations.
Juninho, 37, will face the tribunal along with Santos full-back Juan and Fluminense goalkeeper Ricardo Berna. The three players returned to their sides' changing-rooms before attending post-match doping controls, violating the required controlled conditions in the process.
The former Brazil international will have his case heard on Friday, with the incident relating to Vasco's fixture against Ponte Preta on September 12.
Berna's case will be heard at the same time, while Juan will have his case heard on Tuesday.
They could face bans of up to two years from if found guilty under Articles 2.3 and 2.5 of the World Anti-Doping Code, which refer to "refusal or escaping collection" and "attempting to change any component of the control".
The players will also be accused of breaking Article 258 of the Code of Brazilian Sports Justice, "conduct contrary to the ethics of sport", for which a ban of six matches is possible.
Their clubs also face charges of failing to comply with a legal obligation and could be fined up to $100,000 if found to be complicit in a player's missed test.
Juninho moved back to his homeland for his second spell at Vasco da Gama in 2011.