Toluca's 100% record in the Mexican Apertura came to an end as they were held 1-1 away at Tigres Monterrey.
The leaders, with five wins out of five heading into the match, raced ahead after six minutes when Lucas Silva sent a searing drive from 30 yards into the far left top corner.
Tigres were level just five minutes later though, with Lucas Lobos heading in from eight yards.
Tijuana stand second in the table after a 1-0 win at Club America, their winner coming from Cristian Pellerano who took a touch before lashing a looping shot from 30 yards over goalkeeper Hugo Gonzalez.
Christian Benitez might have equalised in stoppage time for Club America, who had Paul Aguilar sent off after 74 minutes, but the striker failed to convert a penalty, instead blasting his shot over the crossbar.
The match between Pachuca and Santos Laguna finished goalless.