Burton Albion claimed the only upset on FA Cup second round day as a fifth minute goal from Calvin Zola proved enough to give the League Two outfit a 1-0 away to League One strugglers Crewe.
Fellow League Two side Port Vale had looked set to join them in the headlines with Tom Pope's 33rd minute strike putting Micky Adams' men within sight of a famous win over Sheffield United at Bramall Lane.
But the Blades dealt a double blow to Vale's dreams of an upset with Shaun Miller heading an equaliser in the final minute then going on to net an injury-time winner to give his side a dramatic 2-1 win.
Conference National side Luton are the first non-league side guaranteed a place in the third round draw after goals from Andre Gray and Alex Lawless gave the Hatters a 2-1 win over Conference South Dorchester at Kenilworth Road.
Harrogate Town and Hastings Town will join Luton in the hat after playing out a 1-1 draw at Wetherby Road. Tom Platt's header put Harrogate ahead after 41 minutes before Jamie Crellin equalised for the Ryman Premier visitors.
And Conference National pair Lincoln and Mansfield must do it all again in their bid to join the big guns after a 3-3 draw at Sincil Bank. Matthew Rhead's injury-time equaliser gave the visitors a second chance at reaching round three.
Chelmsford's Cup dream came to an end in a 3-0 defeat at Crawley. Nicky Adams, Billy Clarke and Graham Alexander scored for the League Two side, while the visitors had Donovan Simmonds sent off in the first half.
Liam Cooper gave League Two Chesterfield a shock lead against League One leaders Tranmere at Prenton Park, but goals from Cole Stockton and Adam McGurk rescued a 2-1 win for Rovers.
League Two leaders Gillingham were not so fortunate, falling 2-0 at League One Preston , whose goals came in the first half from Jeffrey Monakana and Stuart Beavon.
Oxford United claimed a second chance against Accrington Stanley after a dramatic 3-3 draw with four goals coming in the last 10 minutes. Lee Molyneux seemed to have won it for Stanley in injury-time, but there was still time for Michael Raynes to hit a leveller.
Gary McSheffrey's 38th minute penalty paved the way for Coventry City's 2-1 win over League Two Morecambe at the Ricoh Arena, while a late double from Matt Derbyshire ensured Oldham's progression with a 3-1 win over Doncaster.
Danny Hylton scored twice as struggling Aldershot claimed a surprise 3-2 win at League Two rivals Fleetwood, while Bournemouth's long trip to Carlisle proved worthwhile as Wes Fogden's early goal set up a 3-1 away win.
League Two Rotherham earned a second crack at Notts County after holding the League One side to a 1-1 home draw, while Bury face a midweek replay at Southend after they were deadlocked by the same score at Gigg Lane.