England midfielder Michael Carrick injured his groin in training on Wednesday and will not play in upcoming Euro qualifiers.
Carrick will return to Manchester United after picking up a "minor groin injury" in the first 20 minutes of training, according to the FA.
The 33-year-old missed the start of United's season with torn ankle ligaments, and only just returned to action. He last played for England in a World Cup qualifier against Poland over a year ago.
England are set to face Slovenia on Saturday in a European qualifier and Scotland next Tuesday in a friendly.
The Three Lions will not replace Carrick on the squad.
Twenty-four hours before his withdrawal, Carrick spoke of his pride at earning a recall to the England squad.
Carrick was named on the standby list for the World Cup but did not go to the tournament even though Alex Oxlade-Chamberlain suffered an injury in a warm-up game against Ecuador.
Despite being overlooked for the tournament, Carrick insisted he never thought about retiring from international duty.
''No, I was never tempted to [retire],'' Carrick told a news conference at St George's Park. ''I am only 33 now so hopefully I have got a few more years left, so it was never something that crossed my mind.
''I think you appreciate it a bit more the older you get and the closer you come towards the end of your career. You push things a bit more and being back now is great.''
Carrick's last cap came just over a year ago when England played Poland in their crucial World Cup qualifier at Wembley. The 31-cap midfielder harbours hopes of representing England in Euro 2016.
''Of course (I want to be there),'' he said. ''I don't think this is my last season. I am enjoying things at the moment and I feel good.''