Accrington will not be able to call upon the vast experience of Nicky Hunt and Danny Webber when they host Cheltenham.
Former Bolton full-back Hunt will be out until the end of September at the earliest after damaging the medial crucial ligament in his knee.
Ex-Sheffield United striker Webber will sit out the next three games following his red card in the 3-0 loss to Bury last weekend.
Stanley, who currently prop up the Sky Bet League Two table, are preparing for three games in the space of a week with the Robins clash coming before Cardiff and Burton visit.
Cheltenham striker Terry Gornell has declared himself fit and ready to face his former club.
The Robins' summer signing from Rochdale has made a quick recovery from a groin injury suffered in the 2-0 defeat at Chesterfield 12 days ago and could return to the starting line-up.
Gornell's comeback boosts manager Mark Yates' attacking options with Jamie Cureton still sidelined with a shoulder injury.
Yates was unhappy with his side's 3-1 home defeat to Plymouth last weekend and could shuffle his pack with winger Zack Kotwica and defender Keith Lowe pushing for starts.