Gary MacKenzie and Jon Otsemobor will return to action for MK Dons when Sheffield Wednesday visit stadium:mk.
Manager Karl Robinson told the media at his pre-match press conference that the defensive duo, both of whom have been sidelined through injury, will play a part in the FA Cup third-round replay.
Centre-half MacKenzie recently returned from a three-month absence following hip surgery, making substitute appearances in the league win at Notts County on New Year's Day and the goalless draw against his old club at Hillsborough nine days ago.
Full-back Otsemobor, who was the injury-time hero in the second round against AFC Wimbledon, has not featured since the 3-2 defeat at Brentford on December 8 due to a groin problem.
Robinson also revealed that he has concerns about the fitness of on-loan midfielder Ryan Harley (groin) and striker Ryan Lowe (illness) ahead of Tuesday night.
Meanwhile, Izale McLeod, who made his first appearance for the Dons in Saturday's 1-1 home draw with Bury since returning to the club, is cup-tied having played in the competition for former club Portsmouth.
Should Robinson's side get through they will set up a fourth-round tie at QPR, who they faced in last season's third round, taking the Premier League side to a replay at Loftus Road where they were narrowly beaten 1-0.
Sheffield Wednesday will be without on-loan Jeremy Helan for the FA Cup third-round replay at MK Dons.
The Manchester City midfielder is ineligible for the Owls having already appeared in the competition for Shrewsbury, where he was on loan earlier in the season.
Fellow midfielder David Prutton will also be missing as he sits out the second of a two-game ban for reaching 10 yellow cards.
Manager Dave Jones has no major new injury or suspension problems, but faces a series of selection posers after making six changes to his starting line-up for Saturday's win at Hull.
Giles Coke returned to midfield for his first league start of the season and is expected to retain his place, while Jose Semedo and Paul Corry could return in Helan's absence.
Gary Madine and Chris O'Grady played no part at the weekend and are also pushing for recalls, while Jones must decide whether to stick with Chris Kirkland in goal - he saved a late penalty at the KC Stadium - or bring back Stephen Bywater.