Cowdenbeath will include new signing Albert Puigdollers Saperas in their squad for the first time when East of Scotland League side Vale of Leithen visit Central Park.
The former Barcelona youth and Atletico Madrid B midfielder is set to get his first taste of action for the Blue Brazil in the Scottish Cup.
However, Pablo Navas, Scott Linton, Lewis Milne and Liam Cusack are all missing through injury.
Player/manager Colin Cameron has issued a warning to his players ahead of the tie against their non-league opponents. He said: ``If they think it is going to be easy we could end up in trouble so we need to approach it in the same manner as our First Division games.
``This is a game we should win if we go about it with the right application. We let ourselves down against Dunfermline last week and have the chance to put that right.''
Vale of Leithen co-manager Kerr Dodds is a major doubt for the cup tie after suffering mild concussion in their 2-1 defeat to Edinburgh City last weekend.
Already missing for the Victoria Park side is former Inverness and Arbroath full-back Darran Thompson who has work commitments.
Dodds' fellow co-manager Fraser Ogilvie said: ``We are all really excited about this game and the players have enjoyed the extra exposure they have been getting this week but it is down to the serious stuff now.
``Cowdenbeath have enjoyed a good start to life in the First Division so it is a real step up in class for us and I am looking forward to seeing how the players handle it.''